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KMID : 0380219900230040478
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Journal of Biochemistry and Molecular Biology
1990 Volume.23 No. 4 p.478 ~ p.485
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Involvement of the Unstable Eicosanoids in Sefl-incativation of Soybean Lipoxygenase During Incubation with Archidonic Acid
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Kim Mee-Ree
Sok Dai-Eun
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Abstract
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Gradual decrease of soybean lipoxygenase activity was observed during the prior incubation with arachidonic acid, while preincubation with linoleic acid or 11,14,17-eicosatrienoic acid had no effect on lipoxygenase activity. Among the lipoxygenation pro-ducts of arachidonic acid, only 15(S)-hydroperoxy eicosatetraenoic acid (15(S)-HPETE) inhibited lipoxygenase in a time-dependent manner. Either further oxygenation products of 15(S)-HPETE or non-enzymatic decomposition products of 15(S)-HPETE had no significant inhibitory effect. The observations that the inhibitory effect of 15(S)-HPETE was transient, and not affected by hydroxy radical scavangers support the assumption that the unstable lipid intermediates produced from further conversion of 15(S)-HPETE may be responsible for the irreversible inhibition of lipoxygenase. In support of the above assumption, 13(S)-hydroperoxy-6, 9, 11-octadecatrienoic acid, which can be further lipoxygenated was also found to inactivate lipoxygenase effectively. However, 13(S)-hydroperoxy-9,11-octadecadienoic acid or 13(S)-hydroperoxy-9,11,15-octadecatrienoic acid was not effective.
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